Introduction: Children admitted to the Paediatric Intensive Care Unit (PICU) with neurological conditions are at high risk of secondary brain injury , which can significantly worsen outcomes. Adherence to evidence-based neuroprotective measures is essential to reduce morbidity and mortality . Nursing officers play a pivotal frontline role in implementing these measures continuously at the bedside; however, variations in knowledge and practice may affect the consistency of care delivery . Objectives: To assess the knowledge and current practices of nursing officers regarding neuroprotective measures in the PICU of the National Hospital, Galle. Methods: A prospective, questionnaire-based clinical audit was conducted among all nursing officers in the PICU. Data were collected using a structured Google Form with 15 items (8 knowledge-based and 7 practice-based) developed in accordance with Brain Trauma Foundation guidelines. Knowledge scores ≥70% and practice scores ≥80% were defined as satisfactory . Results: Thirty nursing officers participated (100% response rate). The mean knowledge score was 73.75%, with 73.3% achieving satisfactory knowledge. Mean practice adherence was 98.1%, with all participants meeting the satisfactory threshold. Notable knowledge gaps were identified in ventilation (46.7%) and glucose management (65%). In contrast, temperaturecontrol, neurological monitoring, head positioning, and seizure management demonstrated high knowledge and excellent practice adherence. Conclusions: PICU nursing officers demonstrated good overall knowledge and excellentadherence to neuroprotective practices. Targeted educational interventions focusing on ventilation and glycaemic management are recommended. A followup re-audit is planned to evaluate the effectiveness of these interventions and promote sustained quality improvement in neurocritical care.