Background: Infantile colic is a common condition characterized by excessive crying in infants, often without an identifiable cause. It can significantly impact both infant and parental wellbeing, prompting the need for effective treatments. Objective: To evaluate the efficacy of lactase enzyme (beta-galactosidase) in treating infantile colic in infants aged 1–6 months presenting to a tertiary care hospital. Study Design: A descriptive study . Place and duration of study . 6 months from july 2024 to dec 2024 MTI Hayatabad Medical Complex, Peshawar Methods: This descriptive study was conducted over six months in the outpatient departmentof a tertiary care pediatric unit. A total of 71 infants with infantile colic were enrolled based on the ""3-3-3 rule."" Lactase enzyme drops were administered before each feed. Efficacy was measured by reduction in crying time to