Introduction: Group A rotavirus (RVA) is a leading cause of severe gastroenteritis in children under five years worldwide. Its genetic diversity and continuous evolution pose challenges for timely genotyping. Traditional methods such as nested PCR and Sanger sequencing are timeconsuming, expensive, and operationally complex, limiting their clinical applicability in resourcelimited settings. Objectives: To establish a simple, rapid, and cost-effective genotyping method for RVA G and P genes, and to evaluate its clinical application value. Methods: We designed universal primers and genotype-specific Invader probes targeting common RVA genotypes (G1, G2, G3, G4, G8, G9, G12, and P[4], P[6], P[8], P[9]) based on analysis of 1,729 global RVA sequences from the past two decades. The assay integrates PCR amplification with Invader reaction and oligonucleotide probe-modified gold nanoparticles (AuNPs) for visual detection. Analytical performance was evaluated using standard plasmids; clinical validation was performed on stool samples and compared with nested PCR and Sanger sequencing. Results: The PCR-Invader-AuNP assay demonstrated excellent specificity with no crossreactivity between genotypes. The entire procedure—from amplification to visual readout—was completed within 2 hours using a standard PCR instrument, with results interpretable by naked eye. The per-test cost was under 10 RMB. In clinical validation, the assay achieved excellent sensitivity and specificity , and concordant with Sanger sequencing. Conclusions: This PCR-Invader-AuNP-based method provides a simple, rapid, and low-cost platform for RVA G/P genotyping. Its visual readout and minimal equipment requirements make it particularly suitable for primary healthcare settings, with strong potential to support RVA epidemiological surveillance and vaccine development.