Introduction: Cytokines and lymphocytes orchestrating the pathophysiological landscape have been well explored in adults but remains understudied in children. Objectives: This study evaluated their potential as biomarkers in diagnosis of pediatric Sjögren's syndrome (pSS). Method: We retrospectively analyzed 14 pediatric patients with pSS who attended our rheumatology clinic over 2 years. Plasma levels of IL-4, IL-5, TNF-α, IFN-γ, TNF-β, IL-6, IL-1β, IL-2, IL- 10, IL-8, IL-22, IL-12p70, IL-17F, and IL-17A, as well as percentages and absolute counts of CD3 + , CD19 + , CD3-CD16 + /CD56 + , CD3 + CD4 + , and CD3 + CD8 + lymphocytes, were measured in 14 pSS patients and 12 healthy controls. Results: IL-8 levels were elevated in pSS patients compared to controls. CD3 + CD8 + lymphocytes were increased, while CD3 + , CD3 + CD4 + , CD19 + , and CD3-CD16 + /CD56 + lymphocytes were decreased in pSS patients. Conclusion: IL-8 may be a potential indicator for the early diagnosis of pSS, together with T-, B-, and NK-cell abnormalities, may contribute to disease pathogenesis. Larger studies are needed to confirm these findings.